Invitrogen™ IFN gamma Polyclonal Antibody, Biotin

Catalog No.	Quantity
PIPA184955	50 μg

Catalog No. PIPA184955 Supplier Invitrogen™ Supplier No. PA184955

Description

Rabbit Polyclonal Antibody

Reconstitute with 0.5 mL PBS containing 0.1% BSA. Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles. For long term storage the addition of 0.09% sodium azide is recommended. This antibody may be used in Western Blotting applications under either reducing or non-reducing conditions. Rabbit anti Human Interferon gamma antibody recognizes human IFN-gamma (Interferon-gamma), a 143 amino acid regulatory cytokine and member of the Type II interferon family.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Antigen: IFN gamma
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.1 mg/mL
• Conjugate: Biotin
• Formulation: PBS with no preservative
• Gene: IFNG
• Gene Accession No.: P01579
• Gene Alias: BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
• Gene Symbols: IFNG
• Host Species: Rabbit
• Immunogen: Recombinant human IFN-gamma.
• Purification Method: Affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3458
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

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