IFN-γ Mouse anti-Human, RB545, Clone: B27, BD Horizon™

Catalog No.	Quantity
BDB569266	25 Tests

Catalog No. BDB569266 Supplier BD Biosciences Supplier No. 569266

Description

Mouse Monoclonal Antibody

The B27 monoclonal antibody specifically binds to human interferon-γ (IFN-γ), a 14-18 kDa glycoprotein containing 143 amino acid residues. IFN-γ is a potent multifunctional cytokine produced by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects through specific binding to the high-affinity IFN-γ receptor complex comprised of IFN-γRα (CD119) and IFN-γRβ subunits. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and anti-tumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ influences the regulation of proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. B27 is a neutralizing antibody. The use of B27 antibody for epitope mapping of human IFN-γ has been described. The B27 antibody has been reported not to bind to denatured IFN-γ.

IFN-γ Monoclonal antibody specifically detects IFN-γ in Human samples. It is validated for Intracellular Staining (Flow Cytometry)

Specifications

• Antigen: IFN-γ
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: B27
• Conjugate: RB545
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: IFNG; Interferon-gamma; Interferon-γ; Type II interferon; MAF
• Host Species: Mouse
• Immunogen: Human IFN-γ Recombinant Protein
• Purification Method: Affinity Purified
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 15978,16176,25712,3458,380793,4049
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
• Form: Purified
• Isotype: IgG1 κ
• Excitation/Emission: Excitation Source: Blue 488 nm, Excitation Max: 496 nm, Emission Max: 545 nm