IFNAR1 Monoclonal Antibody (MAR1-5A3), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
16-594-538	Invitrogen™ 16594538	5 mg
16-594-5025	Invitrogen™ 165945025	25 mg

Description

Description: The MAR1-5A3 monoclonal antibody reacts with mouse IFN alpha/beta receptor subunit 1 (IFNAR-1). The type I IFN receptor subunit 1 is co-expressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs, IFN alpha and IFN beta. Type I IFNs are a group of structurally and functionally related proteins that demonstrate antiviral, antiparasitic, and antiproliferative activities. Applications Reported: The MAR1-5A3 antibody has been reported for use in neutralization of IFNAR1 bioactivity. Applications Tested: The Functional Grade Purified MAR1-5A3 antibody has been tested by LAL assay to verify low endotoxin levels, by bioassay for neutralization of mouse IFNAR-1bioactivity. The MAR1-5A3 antibody at 0.04 μg/mL has been found to inhibit by 50% the biological effects of 0.5 ng/mL mouse IFN-a2 (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in Best Protocols® section. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron,2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-& Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).

Specifications

• Antigen: IFNAR1
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene ID (Entrez): 15975
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: Ifnar1

• Clone: MAR1-5A3
• Applications: Functional Assay, Neutralization
• Conjugate: Functional Grade
• Host Species: Mouse
• Target Species: Mouse
• Gene Accession No.: P33896
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody