Ig, κ Light Chain Mouse Anti-Rat, Brilliant Violet 786, Clone: MRK-1, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 743813

Catalog No. BDB743813

Description

The MRK-1 monoclonal antibody reacts specifically with rat Igs bearing light chains. It does not react with rat immunoglobulin light chains or heavy chains. The MRK-1 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.

Specifications

• Antigen: Ig, κ Light Chain
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Igkc, Ig κ light chain, Ig kappa light chain
• Immunogen: Pooled rat Ig
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Clone: MRK-1
• Conjugate: Brilliant Violet 786
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse IgG₁
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal