IgA Monoclonal Antibody (mA-6E1), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-107-72	Invitrogen™ 12420481	50 μg
50-107-73	Invitrogen™ 12420482	100 μg
50-107-74	Invitrogen™ 12420483	200 μg

Description

Description: The mA-6E1 monoclonal antibody reacts with the heavy chain of mouse IgA. It does not react with other isotypes of antibody, including IgD, IgG or IgM, or with other species isotypes. IgA, which is mainly found as a dimer through association of the J chain, is found in mucous secretions, but not in plasma. Fluorochrome conjugated mA-6E1 is used as a detection secondary for mouse IgA primary antibodies. Applications Reported: This mA-6E1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This mA-6E1 antibody has been tested by flow cytometric analysis as a second step to detect mouse IgA primary antibodies. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgA is an immunoglobulin A antibody that is produced by B cells in response to an antigen. It is a crucial component of the mucosal immune system, which protects the body from pathogens that enter through mucosal surfaces such as the respiratory, gastrointestinal, and urogenital tracts. Mouse IgA is composed of two heavy chains and two light chains, and it is the most abundant immunoglobulin in mucosal secretions. It plays a vital role in neutralizing pathogens and preventing their attachment to mucosal surfaces. Mouse IgA is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

Specifications

• Antigen: IgA
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: IgA; Igh-2; Igha; immunoglobulin heavy constant alpha; ImmunoglobulinA
• Gene Symbols: Igha
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Igha

• Clone: mA-6E1
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P01878
• Gene ID (Entrez): 238447
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.