IgA Rat anti-Mouse, Brilliant Violet 421, Clone: C10-1, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 743293

Catalog No. BDB743293

Description

The C10-1 antibody reacts specifically with mouse IgA of Igh-C[a] /and Igh-C[b] /haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with C10-1 mAb.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: IgA
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: IgA, Igh-2, Igha, Immunoglobulin heavy constant alpha
• Immunogen: Pooled mouse IgA
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Clone: C10-1
• Conjugate: Brilliant Violet 421
• Format: Affinity Purified
• Host Species: Rat
• Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG₁, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal