IgD[b] Mouse anti-Mouse, Brilliant Violet 650, Clone: 217-170, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 742386

Catalog No. BDB742386

Description

The 217-170 monoclonal antibody specifically recognizes mouse IgD of Igh-C[b] /haplotype (eg, C57BL/6, C57BL/10, SJL). It does not react with IgD of Igh-C[a] /or related haplotypes (eg, BALB/c, AKR, CBA, C3H/He, C58, DBA/1, DBA/2, NZB, PL, RIII), or Igh-C[e] /haplotype (eg, A). 217-170 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.

Specifications

• Antigen: IgD[b]
• Clone: 217-170
• Conjugate: Brilliant Violet 650
• Gene Alias: Igh-5b, Ighd, immunoglobulin heavy constant delta
• Immunogen: Not reported
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov