IgD[b] Mouse anti-Mouse, Brilliant Violet 786, Clone: 217-170, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 742388

Catalog No. BDB742388

Description

The 217-170 monoclonal antibody specifically recognizes mouse IgD of Igh-C[b] /haplotype (eg, C57BL/6, C57BL/10, SJL). It does not react with IgD of Igh-C[a] /or related haplotypes (eg, BALB/c, AKR, CBA, C3H/He, C58, DBA/1, DBA/2, NZB, PL, RIII), or Igh-C[e] /haplotype (eg, A). 217-170 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.

Specifications

• Antigen: IgD[b]
• Clone: 217-170
• Conjugate: Brilliant Violet 786
• Gene Alias: Igh-5b, Ighd, immunoglobulin heavy constant delta
• Immunogen: Not reported
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG1 κ