Invitrogen™ IgD Monoclonal Antibody (11-26c (11-26)), NovaFluor™ Yellow 610, eBioscience™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ M030T02Y03A

Catalog No. M030T02Y03A

Description

Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells. Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer . Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all expe...

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

Specifications

• Antigen: IgD
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01881
• Gene Symbols: IGHD
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 380797
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 11-26c (11-26)
• Conjugate: NovaFluor Yellow 610
• Gene: IGHD
• Gene Alias: IGHD; immunoglobulin heavy constant delta
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Format: Liquid