Invitrogen™ IgD Monoclonal Antibody (11-26c (11-26)), Super Bright™ 600, eBioscience™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
63-599-382	Invitrogen™ 63599382	100 μg

Description

Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells. Applications Reported: This 11-26 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 11-26 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples...

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

Specifications

• Antigen: IgD
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: Super Bright 600
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01881
• Gene ID (Entrez): 380797
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 11-26c (11-26)
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Mouse
• Gene: Ighd
• Gene Alias: IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
• Gene Symbols: Ighd
• Product Type: Antibody