Invitrogen™ Mouse IgE ELISA Kit

Description

ELISA

The Mouse Immunoglobulin E (Ms IgE) ELISA quantitates Ms IgE in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IgE. Principle of the method The Mouse IgE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.

Specifications

• Accession Number: N/A
• Assay Range: 0.137 to 100 ng/mL
• Assay Sensitivity: 0.14 ng/mL
• Calibration Range: 0.14 ng/mL to 100 ng/mL
• Description: IgE ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Other Proteins Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of IgE in Samples
• Detectable Analytes: Mouse IgE
• Assay: 96
• Conjugate: HRP
• Detection Method: Colorimetric
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects mouse IgE. Other species not determined.
• Substrate Type: TMB
• Sufficient For: 96 Assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 380792
• Host Species: Mouse
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Sample Volume: Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: N/A
• Target: Mouse IgE
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins