Invitrogen™ IGF2 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P12BT50UG

Catalog No. 500P12BT50

Description

AA Sequence of recombinant protein: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-II. Anti-Human IGF-II-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-II (500-P12) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P12BT-1MG will be provided as 2 x 500 μg

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.

Specifications

• Antigen: IGF2
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: IGF2
• Gene Alias: AL033362; C11orf43; FLJ22066; FLJ44734; GRDF; H-IGF-2; IGF; Igf2; Igf-2; IGFII; Igf-II; Insulin like growth factor; insulin like growth factor 2; insulin-like growth factor 2; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor II (IGF-II); Insulin-like growth factor II (somatomedin A); Insulin-like growth factor II Ala-25 Del; insulin-like growth factor II isoform 2 variant 4 preprotein; insulin-like growth factor type 2; M6pr; Mpr; MSA; multiplication-stimulating activity; Multiplication-stimulating polypeptide; Peg2; PP1446; PP9974; preptin; RNIGF2; Somatomedin-A; T3M-11-derived growth factor
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3481
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P01344
• Gene Symbols: IGF2
• Immunogen: E.coli-derived Recombinant Human IGF-II.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody