Invitrogen™ IGFBP5 Human ELISA Kit

Sandwich ELISA Kit

Catalog Number	Mfr. No.	Quantity
EHIGFBP5	Invitrogen™ EHIGFBP5	96 Tests
EHIGFBP5X5	Invitrogen™ EHIGFBP5X5	5 x 96 Tests

Description

The Human Insulin-like Growth Factor Binding Protein 5 (Hu IGFBP5) ELISA quantitates Hu IGFBP5 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IGFBP5. Principle of the method The Human IGFBP5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The insulin-like growth factor-binding proteins, or IGFBPs, are a family of homologous proteins that have co-evolved with the IGFs. They serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system. Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, as well as their concentration and distribution in the extracellular environment. In addition, the IGFBPs appear to have biological activity independent of the IGFs. Seven IGFBPs have thus far been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors. For instance, IGFBP1 is negatively regulated by insulin production. The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes. It has been suggested that IGFBP2 functions as chaperone, escorting IGFs to their target tissues. It is expressed in several human tissues including fetal eye and fetal brain. IGFBP3 is the most abundant IGFBP and is complexed with roughly 80% of the serum IGFs. Both IGFBP3 and IGFBP4 are released by dermal fibroblasts in response to incision injury. IGFBP5 is secreted by myoblasts and may play a key role in muscle differentiation.

Specifications

• Accession Number: P24593
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 3488
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Signal Transduction
• Synonym: IBP5
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 2 ng/mL
• Conjugate: Biotin
• Protein Family: Growth Factors & Receptors
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IBP5
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.