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IgG1/IgG2a Mouse Anti-Rat, Brilliant Violet 711, Clone: G28-5, BD Optibuild™

Catalog No. BDB743853 Shop All BD Biosciences Products
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BDB743853 50 μg
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Catalog No. BDB743853 Supplier BD Biosciences Supplier No. 743853
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Description

Mouse Monoclonal Antibody

The G28-5 monoclonal antibody reacts specifically with rat IgG1 and IgG2a. It does not react with other Ig isotypes. A suspension of pooled rat IgG1 was used as the source of immunogen.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IgG1/IgG2a
Classification Monoclonal
Clone G28-5
Concentration 0.2mg/mL
Conjugate Brilliant Violet 711
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias IgG1/IgG2a, Ig gamma-1/2a, Immunoglobulin gamma 1/2a
Host Species Mouse
Immunogen Pooled Rat IgG
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a
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