IgG1/IgG2a Mouse Anti-Rat, BUV395, Clone: G28-5, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 743855

Catalog No. BDB743855

Description

The G28-5 monoclonal antibody reacts specifically with rat IgG1 and IgG2a. It does not react with other Ig isotypes. A suspension of pooled rat IgG1 was used as the source of immunogen.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: IgG1/IgG2a
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: IgG1/IgG2a, Ig gamma-1/2a, Immunoglobulin gamma 1/2a
• Immunogen: Pooled Rat IgG
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Clone: G28-5
• Conjugate: Brilliant Ultraviolet 395
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse IgG_2a
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal