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IgG1 Rat anti-Mouse, Brilliant Violet 711, Clone: X56, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 742479

Catalog No. BDB742479


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Description

Description

The X56 monoclonal antibody specifically binds to mouse IgG1 of Igh-C[a] /and Igh-C[b] /haplotypes. It does not crossreact with other Ig isotypes.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.
Specifications

Specifications

IgG1
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Igh-4, Ighg1, Immunoglobulin heavy constant gamma 1
Mouse IgG1, κ Antibody
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Murine
X56
Brilliant Violet 711
Affinity Purified
Rat
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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