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IgG2a/IgG2b Rat anti-Mouse, Brilliant Violet 650, Clone: R2-40, BD Optibuild™

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BDB744295 50 μg
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Catalog No. BDB744295 Supplier BD Biosciences Supplier No. 744295
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Description

Rat Monoclonal Antibody

The R2-40 monoclonal antibody specifically recognizes a common epitope, probably located in the CH1 domain, shared by mouse IgG2a, and IgG2b, of Igh-Ca /and Igh-Cb /haplotypes. It does not react with other Ig isotypes.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IgG2a/IgG2b
Classification Monoclonal
Clone R2-40
Concentration 0.2mg/mL
Conjugate Brilliant Violet 650
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias IgG2a (Ighg2a, Igh-1), IgG2b (Ighg2b, Igh-3, gamma2b)
Host Species Rat
Immunogen Pooled Mouse Ig
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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