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IgG2a/IgG2b Rat anti-Mouse, Brilliant Violet 711, Clone: R2-40, BD Optibuild™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 744296

Catalog No. BDB744296


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Description

Description

The R2-40 monoclonal antibody specifically recognizes a common epitope, probably located in the CH1 domain, shared by mouse IgG2a, and IgG2b, of Igh-Ca /and Igh-Cb /haplotypes. It does not react with other Ig isotypes.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

IgG2a/IgG2b
R2-40
Brilliant Violet 711
IgG2a (Ighg2a, Igh-1), IgG2b (Ighg2b, Igh-3, gamma2b)
Pooled Mouse Ig
50 μg
Primary
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Rat
Affinity Purified
RUO
Mouse
IgG1 κ
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