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Rat IgG2a kappa, FITC, clone: eBR2a, Isotype Control, eBioscience™ 
Rat Isotype Control
$54.00 - $217.00
Specifications
| Antigen | Rat IgG2a kappa |
|---|---|
| Clone | eBR2a |
| Target Species | Not Applicable |
| Conjugate | FITC |
| Isotype | IgG2a, kappa |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|
50-186-05
|
Invitrogen
11432141 |
25 Tests |
Each for $86.00
|
|
50-186-06
|
Invitrogen
11432142 |
100 Tests |
Each for $151.00
|
|
50-186-07
|
Invitrogen
11432180 |
25μg |
Each for $54.00
|
|
50-186-10
|
Invitrogen
11432185 |
500μg |
Each for $217.00
|
|
50-112-2122
|
Invitrogen
11432181 |
50μg |
Each for $87.00
|
|
50-112-9602
|
Invitrogen
11432182 |
100μg |
Each for $141.00
|
|
Description
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: The FITC conjugated rat IgG2a isotype control is offered in 2 formats: - µg size: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at 5 µL for the catalog number ending in -41 or -42 and 20µL/test for the product ending in -73 per test. Each test size contains 0.5 µg.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2µm post-manufacturing filtered.Specifications
| Rat IgG2a kappa | |
| Not Applicable | |
| IgG2a, kappa | |
| Monoclonal | |
| Control | |
| PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
| RUO |
| eBR2a | |
| FITC | |
| Control, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry | |
| Liquid | |
| Isotype Control | |
| 4° C, store in dark, DO NOT FREEZE! |
For Research Use Only.
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