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IgG2a Rat anti-Mouse, Brilliant Violet 510, Clone: R19-15, BD Optibuild™

Catalog No. BDB744531 Shop All BD Biosciences Products
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BDB744531 50 μg
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Catalog No. BDB744531 Supplier BD Biosciences Supplier No. 744531
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Description

Rat Monoclonal Antibody

The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] /allotype and weaker reactivity to Igh-I[b] /. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] /allele, which encodes IgG2a[b] /, /is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IgG2a
Classification Monoclonal
Clone R19-15
Concentration 0.2mg/mL
Conjugate Brilliant Violet 510
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias Ighg2a, Immunoglobulin gamma-2a heavy chain, Igh-1, Igh-1a
Host Species Rat
Immunogen Pooled BALB/c and C57BL/6 mouse Ig
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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