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IgG2a Rat anti-Mouse, Brilliant Violet 711, Clone: R19-15, BD Optibuild™

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BDB744533 50 μg
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Catalog No. BDB744533 Supplier BD Biosciences Supplier No. 744533
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Description

Rat Monoclonal Antibody

The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] /allotype and weaker reactivity to Igh-I[b] /. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] /allele, which encodes IgG2a[b] /, /is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IgG2a
Classification Monoclonal
Clone R19-15
Concentration 0.2mg/mL
Conjugate Brilliant Violet 711
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias Ighg2a, Immunoglobulin gamma-2a heavy chain, Igh-1, Igh-1a
Host Species Rat
Immunogen Pooled BALB/c and C57BL/6 mouse Ig
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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