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IgG2b Rat anti-Mouse, Brilliant Violet 510, Clone: R12-3, BD Optibuild™

Catalog No. BDB743175 Shop All BD Biosciences Products
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BDB743175 50 μg
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Catalog No. BDB743175 Supplier BD Biosciences Supplier No. 743175
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Description

Rat Monoclonal Antibody

The R12-3 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of Igh-C[a] /and Igh-C[b] /haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IgG2b
Classification Monoclonal
Clone R12-3
Concentration 0.2mg/mL
Conjugate Brilliant Violet 510
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias Igh-3, gamma2b, Immunoglobulin heavy constant gamma 2B
Host Species Rat
Immunogen Pooled Mouse Ig
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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