IgG2b Rat anti-Mouse, Brilliant Violet 510, Clone: R12-3, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 743175

Catalog No. BDB743175

Description

The R12-3 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of Igh-C[a] /and Igh-C[b] /haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.

Specifications

• Antigen: IgG2b
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Igh-3, gamma2b, Immunoglobulin heavy constant gamma 2B
• Immunogen: Pooled Mouse Ig
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Clone: R12-3
• Conjugate: Brilliant Violet 510
• Format: Affinity Purified
• Host Species: Rat
• Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG_2a, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal