IgM Monoclonal Antibody (II/41), Brilliant Violet™ 711, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
40-757-9080	Invitrogen™ 407579080	25 μg
40-757-9082	Invitrogen™ 407579082	100 μg

Description

The II/41 mAb reacts with theu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome-conjugated II/41 can be used as a detection secondary for mouse IgM. Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis. Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 1ug per test BV 711 is a tandem dye that emits at 713nm and is intended for use on cytometers equipped with a violet (405nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, BV, BUV, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Our internal testing suggests that BV 711 is not compatible with methanol-based fixation. Excitation: 407nm; Emission: 713nm; Laser: Violet Laser. BV is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

Specifications

• Antigen: IgM
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH
• Gene Symbols: Ighm
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Ighm

• Clone: II/41
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 711
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P01872
• Gene ID (Entrez): 16019
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody