Invitrogen™ IgM Monoclonal Antibody (SA-DA4), Super Bright™ 436, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
62999842	Invitrogen™ 62999842	100 Tests

Description

The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells. Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody. Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SA-DA4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product #SB-44...

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

Specifications

• Antigen: IgM
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: Super Bright 436
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01871
• Gene ID (Entrez): 3507
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: SA-DA4
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: IGHM
• Gene Alias: AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH
• Gene Symbols: IGHM
• Product Type: Antibody