Invitrogen™ IL-1 alpha Monoclonal Antibody (40508)

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA523736	Invitrogen™ MA523736	500 μg
PIMA523828	Invitrogen™ MA523828	100 μg

Description

In ELISAs, this antibody shows less than 1% cross-reactivity with recombinant rat (rr) IL-1 alpha /IL-1F1, rpIL-1 alpha /IL-1F1, or rhIL-1 alpha /IL-1F1. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 μg of the antibody by the LAL method.

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Specifications

• Antigen: IL-1 alpha
• Concentration: 0.5 mg/mL
• Applications: ELISA, Neutralization
• Conjugate: Unconjugated
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: P01582
• Gene ID (Entrez): 16175
• Immunogen: E. coli-derived recombinant mouse IL-1 alpha/IL-1F1 Ser115-Ser270.
• Product Type: Antibody

• Clone: 40508
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Classification: Monoclonal
• Form: Lyophilized
• Isotype: IgG2b
• Target Species: Mouse
• Gene: Il1a
• Gene Alias: CTLA8; hematopoietin-1; IL 1 a; IL 1a; IL1; IL-1 alpha; IL17; IL1A; Il-1a; IL-1alpha; IL1-ALPHA; IL1F1; IL1a; ILN; Interleukin; interleukin 1 alpha; interleukin 1, alpha; interleukin 17; interleukin 1a; interleukin 1-alpha; interleukin 1-alpha (AA 1 - 152); Interleukin1 alpha; interleukin-1 alpha; interleukin-1 alpha precursor; Interleukin-17A; preinterleukin 1 alpha; pre-interleukin-1 alpha; pro-interleukin-1-alpha; RP23-160G19.8
• Gene Symbols: Il1a
• Primary or Secondary: Primary
• Purification Method: Protein A/G