Invitrogen™ IL-1 alpha Monoclonal Antibody (ILA8-H12), Biotin

Catalog No.	Quantity
PIM420AB	250 μg

Catalog No. PIM420AB Supplier Invitrogen™ Supplier No. M420AB

Description

Mouse Monoclonal Antibody

M420AB targets IL-1 alpha in ELISA applications and shows reactivity with Human samples. The M420AB immunogen is recombinant human IL-1 alpha. M420AB detects IL-1 alpha which has a predicted molecular weight of approximately 18 kDa. The M420AB IL1a antibody (biotinylated conjugate of clone B133.5) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M700A (clone 2G1). Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Biotinylated antibody M420AB (clone ILA8-H12) and antibody M421A (clone ILA9-H18) have successfully been used in combination with recombinant IL1 alpha protein SIL1A in ELISA applications.

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Specifications

• Antigen: IL-1 alpha
• Applications: ELISA
• Classification: Monoclonal
• Clone: ILA8-H12
• Concentration: 0.5 mg/mL
• Conjugate: Biotin
• Formulation: PBS with 4% BSA and proprietary preservative
• Gene: Il1a
• Gene Accession No.: P01583
• Gene Alias: CTLA8; hematopoietin-1; IL 1 a; IL 1α; IL1; IL-1 alpha; IL17; IL1A; Il-1a; IL-1alpha; IL1-ALPHA; IL1F1; IL1α; ILN; Interleukin; interleukin 1 alpha; interleukin 1, alpha; interleukin 17; interleukin 1a; interleukin 1-alpha; interleukin 1-alpha (AA 1 - 152); Interleukin1 alpha; interleukin-1 alpha; interleukin-1 alpha precursor; Interleukin-17A; preinterleukin 1 alpha; pre-interleukin-1 alpha; pro-interleukin-1-alpha; RP23-160G19.8
• Gene Symbols: Il1a
• Host Species: Mouse
• Immunogen: Recombinant human IL-1 alpha.
• Purification Method: Protein G
• Quantity: 250 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3552
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Safety and Handling

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