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IL-1 alpha Polyclonal Antibody, Rockland™
Description
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1a in the same formats using similar dilutions. The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples. Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form. The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly. The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell. This antibody is also useful for neutralization of human and primate IL-1a activity in bioassays. It does not neutralize the biological activity IL-1b. It does not neutralize the biological activity of mouse, rat or rabbit IL-1a. It will neutralize primate IL-a. For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Specifications
Specifications
| Antigen | IL-1 alpha |
| Applications | ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot |
| Classification | Polyclonal |
| Concentration | 1 mg/mL |
| Conjugate | Unconjugated |
| Formulation | 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2 |
| Gene | Il1a |
| Gene Accession No. | P01583 |
| Gene Alias | BOS_11618; CTLA8; Hematopoietin-1; IL 1 a; IL 1a; IL1; IL-1 alpha; IL17; IL1A; IL-1A; IL-1alpha; IL1-ALPHA; IL1F1; IL1a; ILN; Interleukin; interleukin 1 alpha; interleukin 1, alpha; interleukin 17; interleukin 1-alpha; Interleukin1 alpha; Interleukin-1 alpha; Interleukin-17A; preinterleukin 1 alpha; pro-interleukin-1-alpha; RP23-160G19.8 |
| Gene Symbols | Il1a |
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