IL-1 beta Monoclonal Antibody (A7A10), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA550586

Catalog No. FEMA550586

Description

Positive control: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate. Predicted band size: 31 kDa Subcellular Location: Extracellular exosome, Secreted, Lysosome, Cytosol. Product MA5-50586 was used in an Indirect ELISA.

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

Specifications

• Antigen: IL-1 beta
• Classification: Monoclonal
• Concentration: 2 mg/mL
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P01584
• Gene Symbols: IL1B
• Immunogen: Recombinant protein within Human IL-1 beta aa 117-269/269.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Form: Liquid

• Applications: ELISA, Western Blot
• Clone: A7A10
• Conjugate: Unconjugated
• Gene: IL1B
• Gene Alias: catabolin, cytokine, Hematopoietin 1 (H1), IFN beta inducing factor, il 1b, IL 1β, IL-1, IL1 B, IL-1 beta, IL-1 precursor, IL1B, IL-1B, IL1B1, IL1beta, IL-1beta, IL1-BETA, IL1F2, IL1β, ILN, Interleukin, interleukin 1 beta, Interleukin 1 beta precursor, interleukin 1, beta, interleukin 1, beta 1, interleukin 1-beta, interleukin*1*beta, Interleukin1 beta, interleukin-1 beta, interleukin-1 beta precursor, interleukin-1 beta precursor (AA -113 to 153), interleukin-1 beta proprotein, interleukin-1b, interleukin-1beta, interleukin-beta, LAF, lymphocyte proliferation-potentiating factor, Osteoclast activating factor (OAF), preinterleukin 1 beta, Pro interleukin 1 beta, prointerleukin-1 beta, pro-interleukin-1-beta
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 3553
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Isotype: IgG1