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IL-1 beta Polyclonal Antibody, PeproTech®, Invitrogen™
Goat Polyclonal Antibody
$250.00 - $2175.00
Specifications
Antigen | IL-1 beta |
---|---|
Concentration | 0.1-1.0 mg/mL |
Applications | ELISA, Western Blot |
Classification | Polyclonal |
Conjugate | Unconjugated |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
500P21BG50
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Invitrogen™
500P21BG50UG |
50 μg |
Each for $250.00
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500P21BG100
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Invitrogen™
500P21BG100UG |
100 μg |
Each for $319.00
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500P21BG1MG
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Invitrogen™
500P21BG1MG |
1 mg |
Each for $2,175.00
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Description
AA Sequence of recombinant protein: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human IL-1beta. Anti-Human IL-1beta-specific antibody was purified by affinity chromatography employing an immobilized Human IL-1beta matrix. Sandwich ELISA: To detect Human IL-1beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-1beta (500-P21BGBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-1beta. Western Blot: To detect Human IL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.Specifications
IL-1 beta | |
ELISA, Western Blot | |
Unconjugated | |
Goat | |
Human | |
P01584 | |
IL1B | |
Primary | |
-20°C |
0.1-1.0 mg/mL | |
Polyclonal | |
Lyophilized | |
RUO | |
PBS with no preservative | |
3553 | |
E.coli-derived Recombinant Human IL-1beta. | |
Antigen affinity chromatography | |
IL1B |
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