Novus Biologicals™ IL-1 RII Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IL-1 RII.

Specifications

• Assay Range: 9.375 pg/mL
• Assay Sensitivity: 9.38-600 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IL-1 RII.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: beta, CD121b, CD121b antigen, IL1RBCD121 antigen-like family member B, IL-1R-beta, IL-1RT2, IL-1RT-2, interleukin 1 receptor, type II, Interleukin-1 receptor beta, MGC47725
• Gene ID (Entrez): 7850
• Gene Symbol: IL1R2
• Host Species: Mouse/Rabbit
• Kit Contents:
  Detection Antibody: 0.5 mg/mL mouse anti-IL-1 RII monoclonal antibody conjugated to horseradish-peroxidase (HRP).
  Capture Antibody: 0.5 mg/mL of rabbit anti-IL-1 RII monoclonal antibody.
  Standard: Each vial contains 24 ng of recombinant IL-1 RII.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Phospho Specific, Signal Transduction
• Storage Requirements: Storage is content dependent.
• Target: IL-1 RII
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2.0 μg/mL in CBS before coating 2. Detection Antibody: Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.