Invitrogen™ Porcine IL-12 (IL-12A) ELISA Kit

ELISA

Supplier:  Invitrogen™ ESIL12AX10

Catalog No. 50-254-5275

Description

Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.

Specifications

• Accession Number: Q29053
• Assay Sensitivity: 2.2 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL12A
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hrs 20 mins

• Assay Range: 2.458 to 600 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): 397053
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Target: IL-12 p35
• Total Assay Time: 4 hrs 45 mins