Invitrogen™ IL-12 p35 Monoclonal Antibody (B-T21 (BT21)), eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-8691	Invitrogen™ 14712882	100 μg

Description

Description: The B-T21 antibody reacts with the p35 subunit of human interleukin-12 (IL-12). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated B cells. For optimal detection of human IL-12 p70 by ELISA, a combination of purified B-T21 antibody for capture and biotinylated C8.6 antibody for detection is recommended. Applications Reported: The B-T21 antibody has been reported for use as capture antibody in an IL-12 p70 sandwich ELISA. Applications Tested: The B-T21 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the biotinylated C8.6 (13-7129) antibody for detection and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.

Specifications

• Antigen: IL-12 p35
• Concentration: 0.5 mg/mL
• Applications: ELISA
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P29459
• Gene ID (Entrez): 3592
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography

• Clone: B-T21 (BT21)
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1
• Target Species: Human
• Gene: IL12A
• Gene Alias: CLMF; CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; il 12; IL-12 subunit p35; IL-12, subunit p35; Il12a; IL-12A; IL12p35; IL-12p35; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12 p35 subunit; interleukin 12, p35; interleukin 12a; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Interleukin12; interleukin-12 alpha chain; interleukin-12 beta p35 subunit; interleukin-12 p35 subunit; interleukin-12 subunit alpha; Ll12a; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; P35
• Gene Symbols: IL12A
• Product Type: Antibody