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PeproTech IL-13 Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P178100
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500P178100 100 μg
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Catalog No. 500P178100 Supplier PeproTech Supplier No. 500P178100UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-13. Anti-Murine IL-13-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-13 matrix. Sandwich ELISA: To detect Murine IL-13 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-13 (500-P178Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-13. Western Blot: To detect Murine IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IL-13
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene Il13
Gene Accession No. P20109
Gene Alias ALRH; ALRH antibody; BHR1; il 13; Il13; Il-13; IL-13; cytokine; ILN; Interleukin; interleukin 13; Interleukin13; interleukin-13; interleukin-13 precursor; L13; MGC116786; MGC116788; MGC116789; NC30; NC30 antibody; P600; P600 antibody; T-cell activation protein P600
Gene Symbols Il13
Host Species Rabbit
Immunogen E.coli-derived Recombinant Murine IL-13.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 16163
Target Species Mouse
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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