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Invitrogen™ IL-17A Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ 500P265BT25UG

Description
AA Sequence of recombinant protein: AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-17A. Anti-Murine IL-17A-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect mIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-17A (500-P265) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-17A. Western Blot: To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P265BT-1MG will be provided as 2 x 500 μg
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
Specifications
IL-17A | |
Polyclonal | |
Biotin | |
IL17A | |
Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A | |
Rabbit | |
Antigen Affinity Chromatography | |
RUO | |
16171 | |
-20°C | |
Lyophilized |
ELISA, Western Blot | |
0.1-1.0 mg/mL | |
PBS with no preservative | |
Q62386 | |
IL17A | |
E.coli-derived Recombinant Murine IL-17A. | |
25 μg | |
Primary | |
Mouse | |
Antibody |
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