Novus Biologicals™ IL-18/IL-1F4 Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human IL-18/IL-1F4.

Specifications

• Assay Range: 31.25 pg/mL
• Assay Sensitivity: 31.25-2000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human IL-18/IL-1F4.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: Iboctadekin, IFN-gamma-inducing factor, IGIFIL-1 gamma, IL-18MGC12320, IL1F4iboctadekin, IL-1g, Interferon gamma-inducing factor, interleukin 18 (interferon-gamma-inducing factor), Interleukin-1 gamma, interleukin-18
• Gene ID (Entrez): 3606
• Gene Symbol: IL18
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human IL-18/IL-1F4 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of rabbit anti-Human IL-18/IL-1F4 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 58 ng of recombinant Human IL-18/IL-1F4.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: IL-18/IL-1F4
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.