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Novus Biologicals™ IL-18/IL-1F4 Antibody Pair [HRP]
SDP

Catalog No. NB010025 Shop All R&D Systems Products
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NB010025 15 Plates
NB010026 5 Plates
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Catalog No. NB010025 Supplier Novus Biologicals™ Supplier No. NBP27949315PLATES
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Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse IL-18/IL-1F4.

Specifications

Assay Range 31.25 pg/mL
Assay Sensitivity 31.25-2000 pg/mL
Assay Sandwich ELISA
Conjugate HRP
Product Type Antibody Pairs
Specificity This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse IL-18/IL-1F4.
Target Species Mouse
For Use With (Application) Sandwich ELISA
Gene Alias Iboctadekin, IFN-gamma-inducing factor, IGIFIL-1 gamma, IL-18MGC12320, IL1F4iboctadekin, IL-1g, Interferon gamma-inducing factor, interleukin 18 (interferon-gamma-inducing factor), Interleukin-1 gamma, interleukin-18
Gene ID (Entrez) 3606
Gene Symbol IL18
Host Species Rabbit
Kit Contents
  • Detection Antibody: 0.2 mg/mL of rabbit anti-IL-18/IL-1F4 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  • Capture Antibody: 1 mg/mL of rabbit anti-Mouse IL-18/IL-1F4 monoclonal antibody (in PBS, pH 7.4).
  • Standard: Each vial contains 60 ng of recombinant Mouse IL-18/IL-1F4.
  • Quantity 15 Plates
    Regulatory Status RUO
    Storage Requirements Storage is content dependent.
    Target IL-18/IL-1F4
    Technique 1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.125 μg/mL in Dilution Buffer before use. 3. Standard: Reconstitute with 1 ml Dilution Buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2000 pg/mL is recommended.
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