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IL-2 Rat anti-Human, Rhesus, Cynomolgus, Baboon, Brilliant Violet 750, Clone: MQ1-17H12, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 566361

Catalog No. BDB566361


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Description

Description

The MQ1-17H12 monoclonal antibody specifically binds to the multifunctional cytokine, human Interleukin-2 (IL-2). IL-2 is produced by activated T cells and has multiple functions that can affect the growth, proliferation, differentiation and survival of many different target cell types including T cells, B cells, NK cells, monocytes and macrophages. The immunogen used to generate the MQ1-17H12 hybridoma was purified recombinant human IL-2 protein. The MQ1-17H12 antibody reportedly neutralizes the biological activity of human IL-2.

The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 750nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. BV750
Specifications

Specifications

IL-2
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
IL2, Interleukin-2, T-cell growth factor, TCGF
Human IL-2 Recombinant Protein
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV750 under optimum conditions, and unconjugated antibody and free BD Horizon BV750 were removed.
RUO
Primary
Baboon, Cynomolgus, Human, Rhesus
MQ1-17H12
Brilliant Violet 750
Affinity Purified
Rat
Rat IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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