Invitrogen™ IL-21 Monoclonal Antibody (eBio2B2-G20 (2B2-G20)), Biotin, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 13721881

Catalog No. 50-121-92

Description

Description: The monoclonal antibody eBio2B2-G20 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15. Applications Reported: The eBio2B2-G20 antibody has been reported for use in ELISA detection of IL-21 analysis. Applications Tested: The eBio2B2-G20 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-21 (IL-21) in combination with the purified eBio3A3-N2 (Product # 14-7219-82) antibody for capture and recombinant human IL-21 (Product # 14-8219) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 60 pg/mL should be included in each ELISA plate. Filtration: 0.2 μm post-manufacturing filtered.

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

Specifications

• Antigen: IL-21
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9HBE4
• Gene Symbols: IL21
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 59067
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: ELISA
• Clone: eBio2B2-G20 (2B2-G20)
• Conjugate: Biotin
• Gene: IL21
• Gene Alias: CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

Safety and Handling

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