Invitrogen™ IL-21 Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA546962

Catalog No. PIPA546962

Description

In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL-2, rmIL-4, rmIL-15, and recombinant human IL-21 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is <0.30 EU per 1 μg of the antibody by the LAL method.

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

Specifications

• Antigen: IL-21
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IL21
• Gene Alias: CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 60505
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: ELISA, Neutralization, Western Blot, Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q9ES17
• Gene Symbols: IL21
• Immunogen: E. coli-derived recombinant mouse IL-21 Pro25-Ser146.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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