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IL-21 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

$250.00 - $2175.00

Specifications

Antigen IL-21
Concentration 0.1-1.0 mg/mL
Applications ELISA, Western Blot
Classification Polyclonal
Conjugate Biotin
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
500P278BT25
Encompass_Preferred
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Invitrogen™
500P278BT25UG
25 μg
Each for $250.00
Only null left
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500P278BT50
Encompass_Preferred
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Invitrogen™
500P278BT50UG
50 μg
Each for $319.00
Only null left
Edge
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50-271-8543
Encompass_Preferred
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Invitrogen™
500P278BT500UG
500 μg
Each for $2,175.00
Only null left
Add to Cart
 
Description

Description

AA Sequence of recombinant protein: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-21. Anti-Murine IL-21-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect mIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-21 (500-P278) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-21. Western Blot: To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-21 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P278BT-1MG will be provided as 2 x 500 μg

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.
Specifications

Specifications

IL-21
ELISA, Western Blot
Biotin
Rabbit
Mouse
Q9ES17
60505
E.coli-derived Recombinant Murine IL-21.
Antigen Affinity Chromatography
IL21
0.1-1.0 mg/mL
Polyclonal
Lyophilized
RUO
PBS with no preservative
CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11
IL21
Primary
-20°C
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