IL-21 Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 200401B53S

Catalog No. 50-199-0869

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

Specifications

• Antigen: IL-21
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IL21
• Gene Alias: BOS_16376; CVID11; CX2; IL21; IL-21; ILN; Interleukin; interleukin 21; interleukin 21-like; Interleukin21; Interleukin-21; interleukin-21 isoform; LOC100344172; Za11
• Host Species: Rabbit
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Equine, Pig
• Product Type: Antibody

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: Q76LU5, Q76LU6
• Gene Symbols: IL21
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
• Regulatory Status: RUO
• Gene ID (Entrez): 100063803, 378475, 403123
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized