Invitrogen™ IL-21 Recombinant Rabbit Monoclonal Antibody (18H2L4)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ 701111

Catalog No. PI701111

Description

This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

Specifications

• Antigen: IL-21
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: Q9HBE4
• Gene Symbols: IL21
• Immunogen: Recombinant protein corresponding to amino acids 37-162 of human IL-21.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA
• Clone: 18H2L4
• Conjugate: Unconjugated
• Gene: IL21
• Gene Alias: CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 59067
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

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