Invitrogen™ Mouse IL-25/IL-17E ELISA Kit

Catalog No. 50-112-5450

Supplier: Invitrogen™ BMS6046

Description

ELISA

The Mouse Interleukin-25/Interleukin 17E (Ms IL-25/IL-17E) ELISA quantitates Ms IL-25/IL-17E in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-25/IL-17E. Principle of the method The Mouse IL-25/IL-17E solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.

Specifications

• Assay Range: 3.9-250 pg/mL
• Assay Sensitivity: 0.9 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to mouse IL-17E (IL-25)
  Biotin-Conjugate anti-mouse IL-17E (IL-25) monoclonal antibody
  Streptavidin-HRP
  Mouse IL-17E (IL-25) Standard lyophilized, 500 pg/mL upon reconstitution
  Sample Diluent
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: D17Wsu104e; il 17e; Il17e; IL-17E; Il25; IL-25; ILN; Interleukin; interleukin 17E; interleukin 25; interleukin-17E; Interleukin-25; Ly6elg; Mydgf; myeloid derived growth factor; myeloid-derived growth factor; RGD1561632; UNQ3120/PRO10272; UPF0556 protein C19orf10 homologD17Wsu104e; il 17e; Il17e; IL-17E; Il25; IL-25; ILN; Interleukin; interleukin 17E; interleukin 25; interleukin-17E; Interleukin-25; Ly6elg; Mydgf; myeloid derived growth factor; myeloid-derived growth factor; RGD1561632; UNQ3120/PRO10272; UPF0556 protein C19orf10 homolog; IL-17E; Il17e
• Gene ID (Entrez): 140806
• Gene Symbol: IL25
• Interassay CV: 8.8%
• Intraassay CV: 6.5%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2-8°C
• Synonym: Interleukin-17 E, Interleukin-25
• Target: IL-25
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min