Invitrogen™ IL-25 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P89BT50UG

Catalog No. 500P89BT50

Description

AA Sequence of recombinant protein: MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CPHCVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17E. Anti-Human IL-17E-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IL-17E by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-17E (500-P89Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17E. Western Blot: To detect Human IL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL.andnbsp; When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-17E is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P89BT-1MG will be provided as 2 x 500 μg

Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.

Specifications

• Antigen: IL-25
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: IL25
• Gene Alias: D17Wsu104e; il 17e; Il17e; IL-17E; Il25; IL-25; ILN; Interleukin; interleukin 17E; interleukin 25; interleukin-17E; Interleukin-25; Ly6elg; Mydgf; myeloid derived growth factor; myeloid-derived growth factor; RGD1561632; UNQ3120/PRO10272; UPF0556 protein C19orf10 homolog
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 64806
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q9H293
• Gene Symbols: IL25
• Immunogen: E.coli-derived Recombinant Human IL-17E.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody