IL-35 EBI3 Mouse anti-Mouse, Biotin, Clone: V1.4C4.22, Rockland™

Mouse Monoclonal Antibody

Supplier:  Rockland Immunochemicals 210306B66

Catalog No. 50-199-1471

Description

Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is specific for mouse EBI3 protein. A BLAST analysis was used to suggest cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with EBI-3 from other sources has not been determined.

IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.

Specifications

• Antigen: IL-35 EBI3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Gene Accession No.: O35228, Q8K3I6
• Gene Symbols: Ebi3, IL27
• Immunogen: Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
• Regulatory Status: RUO
• Gene ID (Entrez): 246779, 50498
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized

• Applications: ELISA, Immunoprecipitation, Western Blot
• Clone: V1.4C4.22
• Conjugate: Biotin
• Gene: IL27
• Gene Alias: cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus induced gene 3 (similar to Interleukin-27 beta chain precursor); Epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus-induced gene 3 protein homolog; Il27; IL-27; IL27 p28; IL-27 p28 subunit; IL27 subunit; IL-27 subunit alpha; IL-27 subunit beta; Il27a; IL-27A; IL-27-A; IL27-A; IL27B; IL-27B; IL27p28; IL-27p28; IL30; IL35 subunit; ILN; Interleukin; interleukin 27; interleukin 30; Interleukin-27 subunit alpha; Interleukin-27 subunit beta; Interleukin-30; MGC71873; M-IL-27 (p28); p28; RGD1561420
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b