Invitrogen™ IL-38 Monoclonal Antibody (H127C), eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 14738537

Catalog No. 14-738-537

Description

Description: The monoclonal antibody H127C recognizes human IL-38. IL-38 is a member of the Interleukin-1 family (IL-1F) and shares homology with IL-1R antagonist (IL-1Ra) and IL-36R antagonist (IL-36Ra). IL-38 is expressed mostly in the skin and proliferating B cells of the tonsil and is also present at low levels in the heart and placenta. IL-38 functions as an anti-inflammatory cytokine that inhibits IL-36 by binding to IL-36R and also inhibits the production of IL-17 and IL-22. Gene polymorphisms of IL-38 are associated with psoriatic arthritis, ankylosing spondylitis, and heart disease. Applications Reported: This H127C antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, ELISA, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This H127C antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 0.1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

The cytokine IL-1 is responsible for initiating a variety of activities through the activation of transcription factors, NF-kappa B and AP-1, thereby promoting host response to injury or infection. IL-1F10 (Interleukin-1 family member 10), also known as FIL1T, IL1HY2 or FKSG75, is highly expressed in the skin, spleen and proliferating B-cells of the tonsil. IL-1F10 binds soluble IL-1 receptor type 1, and is one of nine Interleukin 1 families clustered on chromosome 2, where it is thought to participate in the regulation of adapted and innate immune responses. IL-1F10 and IL-1RN polymorphisms may play an important role in the susceptibility to developing rheumatoid arthritis.

Specifications

• Antigen: IL-38
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8WWZ1
• Gene Symbols: IL1F10
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 84639
• Content And Storage: 4°C
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: H127C
• Conjugate: Unconjugated
• Gene: IL1F10
• Gene Alias: family of interleukin 1-theta; FIL1 theta; FIL1T; FIL1-theta; FKSG75; IL-1 theta; Il1f10; IL-1F10; IL-1F10 (canonical form IL-1F10a); IL1HY2; IL-1HY2; IL1-theta; IL38; IL-38; Interleukin; interleukin 1 family member 10 (theta); interleukin 1 family, member 10; interleukin 1 family, member 10 (theta); interleukin 1 family, member 7; interleukin-1 family member 10; Interleukin-1 HY2; interleukin-1 receptor antagonist FKSG75; interleukin-1 receptor antagonist-like FIL1 theta; Interleukin-1 theta; Interleukin-38; UNQ6119/PRO20041
• Host Species: Mouse
• Quantity: 2 mg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ