Invitrogen™ IL-4 Monoclonal Antibody (BVD4-1D11)

Catalog No.	Quantity
AMC0042	500 μg

Catalog No. AMC0042 Supplier Invitrogen™ Supplier No. AMC0042

Description

Rat Monoclonal Antibody

IL-4 Monoclonal Antibody for Flow, ELISA, Neu

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Antigen: IL-4
• Applications: ELISA, Flow Cytometry, Neutralization
• Classification: Monoclonal
• Clone: BVD4-1D11
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative
• Gene: Il4
• Gene Accession No.: P07750
• Gene Alias: B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
• Gene Symbols: Il4
• Host Species: Rat
• Immunogen: Recombinant mouse IL-4.
• Purification Method: Purified
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16189
• Target Species: Mouse
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b

Frequently Asked Questions (FAQs)

How does monensin help in intracellular staining of some secreted proteins?

Monensin is an Na+ ionophore. Treatment of cells with monensin disrupts intracellular Na+ and H+ gradients, and thereby inhibits Golgi transport and secretion. In monensin-treated cells, nascently synthesized proteins are retained and concentrated within the cell. This increased protein concentration facilitates detection of specific proteins by the intracellular staining technique.

Treating cells with monensin is especially useful when monitoring the efficacy of stimulation protocols which induce rapid or transient increases in protein synthesis. An example of such a stimulation protocol is the treatment of cells with phorbol myristate acetate (PMA) plus Ca2+ ionophore A23187. This stimulation protocol rapidly up-regulates IL-4 synthesis. We have observed that when isolated human PBMC's are treated concurrently with PMA/A23187 plus monensin, significant levels of IL-4 production are detected within a subpopulation by the intracellular staining technique. However, when the stimulation is performed with PMA/A23187 alone, IL-4 production cannot be detected by this technique.

We have evaluated the effect of the timing of monensin addition on our ability to detect IL-4, plus several other cytokines including IL-2 and INF-gamma. Our experimental results indicate that monensin addition timing should be optimized for the particular stimulation protocol and cytokine under investigation. However, in all cases examined, we find that adding monensin at the beginning of the stimulation protocol allows us to detect higher levels of cytokine than when we omit monensin from the experiment.