Invitrogen™ IL-4 Monoclonal Antibody (E10022), Biotin

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ M2IL4BI

Catalog No. FERM2IL4BI

Description

M2IL4BI targets IL-4 in ELISA applications and shows reactivity with Human samples. The M2IL4BI immunogen is recombinant human IL-4 purified from Pichia pastoris. M2IL4BI detects IL-4 which has a predicted molecular weight of approximately 15 kDa. The M2IL4BI IL4 antibody (biotinylated conjugate of clone E10022) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M3IL4I (clone E10023). Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Biotinylated antibody M2IL4BI (clone E10022) and antibody M3IL4I (clone E10023) have successfully been used in combination with recombinant IL4 protein SIL4 in ELISA applications.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Antigen: IL-4
• Classification: Monoclonal
• Concentration: 0.11 mg/mL
• Formulation: PBS with 10% trehalose and proprietary preservative
• Gene Accession No.: P05112
• Gene Symbols: Il4
• Immunogen: Recombinant human IL-4 purified from Pichia pastoris.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Western Blot
• Clone: E10022
• Conjugate: Biotin
• Gene: Il4
• Gene Alias: B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 3565
• Content And Storage: 4°C
• Form: Lyophilized