IL-6 Rabbit anti-Human, Biotin, Polyclonal, Rockland™

Catalog No.	Quantity
50-199-1424	100 μg

Catalog No. 50-199-1424 Supplier Rockland Immunochemicals Supplier No. 209406310

Description

Rabbit Polyclonal Antibody

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Specifications

• Antigen: IL-6
• Applications: ELISA, Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Biotin
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Gene: Il6
• Gene Accession No.: P05231
• Gene Alias: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BOS_4106; BSF2; BSF-2; CDF; ChIL-6; CTL differentiation factor; EGM_12696; HGF; H-IL-6; HSF; Hybridoma growth factor; hypothetical protein; IFNB2; IFN-beta-2; IL6; Il-6; ILg6; ILN; Interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin BSF-2; Interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 protein; M-IL-6; R-IL-6
• Gene Symbols: Il6
• Host Species: Rabbit
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3569
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Lyophilized