Invitrogen™ IL-7 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P2750UG

Catalog No. 500P2750UG

Description

AA Sequence of recombinant protein: MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-7. Anti-Human IL-7-specific antibody was purified by affinity chromatography employing an immobilized Human IL-7 matrix. Sandwich ELISA: To detect Human IL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-7 (500-P27Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-7. Western Blot: To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.

Specifications

• Antigen: IL-7
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Il7
• Gene Alias: A630026I06Rik; H-IL-7; hlb368; IL7; Il-7; ILN; Interleukin; interleukin 7; Interleukin7; interleukin-7; M-IL-7
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3574, 3575
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P13232, P16871
• Gene Symbols: Il7, IL7r
• Immunogen: E.coli-derived Recombinant Human IL-7.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody